Heterokaryosis and parasexual recombination for the pathogenic challenges off Fusarium oxysponrm
Heterokaryosis and parasexual recombination for the pathogenic challenges off Fusarium oxysponrm
V. Heterokaryosis and parasexuality
Make use of the “0”spot for one of the parents and mention the worries amount towards plate. Make use of the theme with the replicator. Incubate 2-three days. Replicate brand new segregants towards the a series of decide to try dishes playing with a replicator which have, e.g., 21 needles. Draw this new dishes that have a variety. Incubate dos-three days. Rating the exam plates and you may listing the phenotypes throughout the scoring desk. You will need to dictate the fresh ploidy of territories towards the base out of the latest indicators. See the ploidy off undecided territories. Build a listing of the newest genotypes (you need a utility). Dictate brand new part of the latest recombinants to the additional markers. And that markers is connected? Would you come across intrachromosomal recombination? In which linkage category ‘s the unfamiliar marker?
In this try out i influence new gene order and you may venue out-of this new centromere for the linkage category VI ofA. niger.Various techniques for your choice of mitotic recombinants are utilized. New markers involved try: pubA1, pyrB4, c d l . New c d locus is actually critical on chromosome case and you may ergo most appropriate because the options marker. […]